A yeast knockout strain to discriminate between active and inactive tRNA molecules
Here we report the construction of a yeast genetic screen designed to identify essential residues in tRNA(Arg). The system consists of a tRNA(Arg) knockout strain and a set of vectors designed to rescue and select for variants of tRNA(Arg). By plasmid shuffling we selected inactive tRNA mutants that...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2003
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169964/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12907713/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkg685 |
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