A yeast knockout strain to discriminate between active and inactive tRNA molecules

Here we report the construction of a yeast genetic screen designed to identify essential residues in tRNA(Arg). The system consists of a tRNA(Arg) knockout strain and a set of vectors designed to rescue and select for variants of tRNA(Arg). By plasmid shuffling we selected inactive tRNA mutants that...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Geslain, Renaud, Martin, Franck, Camasses, Alain, Eriani, Gilbert
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2003
Assuntos:	Articles
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169964/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12907713/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkg685
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