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Identification of a strong binding site for kinesin on the microtubule using mutant analysis of tubulin
The kinesin-binding site on the microtubule has not been identified because of the technical difficulties involved in the mutant analyses of tubulin. Exploiting the budding yeast expression system, we succeeded in replacing the negatively charged residues in the α-helix 12 of β-tubulin with alanine...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1698889/ https://ncbi.nlm.nih.gov/pubmed/17124495 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.emboj.7601442 |
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