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Intracellular protein interaction mapping with FRET hybrids

A quantitative methodology was developed to identify protein interactions in a broad range of cell types by using FRET between fluorescent proteins. Genetic fusions of a target receptor to a FRET acceptor and a large library of candidate peptide ligands to a FRET donor enabled high-throughput optica...

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Bibliografiske detaljer
Main Authors: You, Xia, Nguyen, Annalee W., Jabaiah, Abeer, Sheff, Mark A., Thorn, Kurt S., Daugherty, Patrick S.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1693684/
https://ncbi.nlm.nih.gov/pubmed/17130455
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0605422103
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