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Directed evolution of protease beacons that enable sensitive detection of endogenous MT1-MMP activity in tumor cell lines
Directed evolution was applied to identify peptide substrates with enhanced hydrolysis rates by MT1-MMP suitable for protease beacon development. Screening of a random pentapeptide library, using two-color CLiPS, yielded several substrates identical to motifs in distinct collagens that shared the co...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3073733/ https://ncbi.nlm.nih.gov/pubmed/21439484 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2010.12.017 |
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