PCR-Restriction Fragment Length Polymorphism Method for Detection of Cyclospora cayetanensis in Environmental Waters without Microscopic Confirmation

We developed an alternative nested-PCR-restriction fragment length polymorphism (RFLP) protocol for the detection of Cyclospora cayetanensis in environmental samples that obviates the need for microscopic examination. The RFLP method, with the restriction enzyme AluI, differentiates the amplified ta...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Shields, Joan M., Olson, Betty H.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2003
Assuntos:	Public Health Microbiology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169151/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12902255/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.8.4662-4669.2003
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