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PCR-Restriction Fragment Length Polymorphism Method for Detection of Cyclospora cayetanensis in Environmental Waters without Microscopic Confirmation
We developed an alternative nested-PCR-restriction fragment length polymorphism (RFLP) protocol for the detection of Cyclospora cayetanensis in environmental samples that obviates the need for microscopic examination. The RFLP method, with the restriction enzyme AluI, differentiates the amplified ta...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2003
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC169151/ https://ncbi.nlm.nih.gov/pubmed/12902255 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.4662-4669.2003 |
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