PCR-Restriction Fragment Length Polymorphism Method for Detection of Cyclospora cayetanensis in Environmental Waters without Microscopic Confirmation

We developed an alternative nested-PCR-restriction fragment length polymorphism (RFLP) protocol for the detection of Cyclospora cayetanensis in environmental samples that obviates the need for microscopic examination. The RFLP method, with the restriction enzyme AluI, differentiates the amplified ta...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Shields, Joan M., Olson, Betty H.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2003
Soggetti:	Public Health Microbiology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169151/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12902255/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.8.4662-4669.2003
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