PCR-Restriction Fragment Length Polymorphism Method for Detection of Cyclospora cayetanensis in Environmental Waters without Microscopic Confirmation

We developed an alternative nested-PCR-restriction fragment length polymorphism (RFLP) protocol for the detection of Cyclospora cayetanensis in environmental samples that obviates the need for microscopic examination. The RFLP method, with the restriction enzyme AluI, differentiates the amplified ta...

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Vydáno v:	Appl Environ Microbiol
Hlavní autoři:	Shields, Joan M., Olson, Betty H.
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 2003
Témata:	Public Health Microbiology
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169151/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12902255/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.8.4662-4669.2003
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