Detection of Bacteria Carrying the stx(2) Gene by In Situ Loop-Mediated Isothermal Amplification

A new in situ DNA amplification technique for microscopic detection of bacteria carrying a specific gene is described. Loop-mediated isothermal amplification (LAMP) was used to detect stxA(2) in Escherichia coli O157:H7 cells. The mild permeabilization conditions and low isothermal temperature used...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Maruyama, Fumito, Kenzaka, Takehiko, Yamaguchi, Nobuyasu, Tani, Katsuji, Nasu, Masao
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2003
Schlagworte:	Methods
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169117/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12902306/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.8.5023-5028.2003
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