16S Ribosomal DNA-Based Analysis of Bacterial Diversity in Purified Water Used in Pharmaceutical Manufacturing Processes by PCR and Denaturing Gradient Gel Electrophoresis

The bacterial community in partially purified water, which is prepared by ion exchange from tap water and is used in pharmaceutical manufacturing processes, was analyzed by denaturing gradient gel electrophoresis (DGGE). 16S ribosomal DNA fragments, including V6, -7, and -8 regions, were amplified w...

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Vydáno v:	Appl Environ Microbiol
Hlavní autoři:	Kawai, Mako, Matsutera, Eiichi, Kanda, Hisashi, Yamaguchi, Nobuyasu, Tani, Katsuji, Nasu, Masao
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 2002
Témata:	Environmental Microbiology and Biodegradation
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC126735/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11823209/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.2.699-704.2002
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