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Detection of Bacteria Carrying the stx(2) Gene by In Situ Loop-Mediated Isothermal Amplification
A new in situ DNA amplification technique for microscopic detection of bacteria carrying a specific gene is described. Loop-mediated isothermal amplification (LAMP) was used to detect stxA(2) in Escherichia coli O157:H7 cells. The mild permeabilization conditions and low isothermal temperature used...
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| Hoofdauteurs: | , , , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
American Society for Microbiology
2003
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC169117/ https://ncbi.nlm.nih.gov/pubmed/12902306 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.5023-5028.2003 |
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