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Detection of Bacteria Carrying the stx(2) Gene by In Situ Loop-Mediated Isothermal Amplification

A new in situ DNA amplification technique for microscopic detection of bacteria carrying a specific gene is described. Loop-mediated isothermal amplification (LAMP) was used to detect stxA(2) in Escherichia coli O157:H7 cells. The mild permeabilization conditions and low isothermal temperature used...

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Bibliografski detalji
Glavni autori: Maruyama, Fumito, Kenzaka, Takehiko, Yamaguchi, Nobuyasu, Tani, Katsuji, Nasu, Masao
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2003
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC169117/
https://ncbi.nlm.nih.gov/pubmed/12902306
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.5023-5028.2003
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