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Cloning of a Nitrilase Gene from the Cyanobacterium Synechocystis sp. Strain PCC6803 and Heterologous Expression and Characterization of the Encoded Protein

The gene encoding a putative nitrilase was identified in the genome sequence of the photosynthetic cyanobacterium Synechocystis sp. strain PCC6803. The gene was amplified by PCR and cloned into an expression vector. The encoded protein was heterologously expressed in the native form and as a His-tag...

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Bibliografski detalji
Izdano u:Appl Environ Microbiol
Glavni autori: Heinemann, Ute, Engels, Dirk, Bürger, Sibylle, Kiziak, Christoph, Mattes, Ralf, Stolz, Andreas
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2003
Teme:
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169084/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12902216/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.8.4359-4366.2003
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