Cloning of a Nitrilase Gene from the Cyanobacterium Synechocystis sp. Strain PCC6803 and Heterologous Expression and Characterization of the Encoded Protein

The gene encoding a putative nitrilase was identified in the genome sequence of the photosynthetic cyanobacterium Synechocystis sp. strain PCC6803. The gene was amplified by PCR and cloned into an expression vector. The encoded protein was heterologously expressed in the native form and as a His-tag...

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Vydáno v:	Appl Environ Microbiol
Hlavní autoři:	Heinemann, Ute, Engels, Dirk, Bürger, Sibylle, Kiziak, Christoph, Mattes, Ralf, Stolz, Andreas
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 2003
Témata:	Physiology and Biotechnology
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169084/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12902216/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.8.4359-4366.2003
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