Cloning of a Nitrilase Gene from the Cyanobacterium Synechocystis sp. Strain PCC6803 and Heterologous Expression and Characterization of the Encoded Protein

The gene encoding a putative nitrilase was identified in the genome sequence of the photosynthetic cyanobacterium Synechocystis sp. strain PCC6803. The gene was amplified by PCR and cloned into an expression vector. The encoded protein was heterologously expressed in the native form and as a His-tag...

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Bibliographic Details
Main Authors: Heinemann, Ute, Engels, Dirk, Bürger, Sibylle, Kiziak, Christoph, Mattes, Ralf, Stolz, Andreas
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2003
Subjects:
Physiology and Biotechnology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC169084/
https://ncbi.nlm.nih.gov/pubmed/12902216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.4359-4366.2003
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