Efficient Library Construction by In Vivo Recombination with a Telomere-Originated Autonomously Replicating Sequence of Hansenula polymorpha

A high frequency of transformation and an equal gene dosage between transformants are generally required for activity-based selection of mutants from a library obtained by directed evolution. An efficient library construction method was developed by using in vivo recombination in Hansenula polymorph...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Kim, So-Young, Sohn, Jung-Hoon, Bae, Jung-Hoon, Pyun, Yu-Ryang, Agaphonov, Michael O., Ter-Avanesyan, Michael D., Choi, Eui-Sung
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2003
Schlagworte:
Methods
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC169078/
https://ncbi.nlm.nih.gov/pubmed/12902228
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.4448-4454.2003
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge