Efficient Library Construction by In Vivo Recombination with a Telomere-Originated Autonomously Replicating Sequence of Hansenula polymorpha

A high frequency of transformation and an equal gene dosage between transformants are generally required for activity-based selection of mutants from a library obtained by directed evolution. An efficient library construction method was developed by using in vivo recombination in Hansenula polymorph...

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Autors principals: Kim, So-Young, Sohn, Jung-Hoon, Bae, Jung-Hoon, Pyun, Yu-Ryang, Agaphonov, Michael O., Ter-Avanesyan, Michael D., Choi, Eui-Sung
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2003
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Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC169078/
https://ncbi.nlm.nih.gov/pubmed/12902228
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.4448-4454.2003
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