Production of succinic acid through overexpression of NAD(+)-dependent malic enzyme in an Escherichia coli mutant.

NAD(+)-dependent malic enzyme was cloned from the Escherichia coli genome by PCR based on the published partial sequence of the gene. The enzyme was overexpressed and purified to near homogeneity in two chromatographic steps and was analyzed kinetically in the forward and reverse directions. The Km...

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Опубліковано в::	Appl Environ Microbiol
Автори:	Stols, L, Donnelly, M I
Формат:	Artigo
Мова:	Inglês
Опубліковано:	American Society for Microbiology (ASM) 1997
Предмети:	Research Article
Онлайн доступ:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168564/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9212416/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.7.2695-2701.1997
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