Production of succinic acid through overexpression of NAD(+)-dependent malic enzyme in an Escherichia coli mutant.
NAD(+)-dependent malic enzyme was cloned from the Escherichia coli genome by PCR based on the published partial sequence of the gene. The enzyme was overexpressed and purified to near homogeneity in two chromatographic steps and was analyzed kinetically in the forward and reverse directions. The Km...
I tiakina i:
| I whakaputaina i: | Appl Environ Microbiol |
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| Ngā kaituhi matua: | , |
| Hōputu: | Artigo |
| Reo: | Inglês |
| I whakaputaina: |
American Society for Microbiology (ASM)
1997
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| Ngā marau: | |
| Urunga tuihono: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168564/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9212416/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.7.2695-2701.1997 |
| Ngā Tūtohu: |
Kāore He Tūtohu, Me noho koe te mea tuatahi ki te tūtohu i tēnei pūkete!
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