Production of succinic acid through overexpression of NAD(+)-dependent malic enzyme in an Escherichia coli mutant.
NAD(+)-dependent malic enzyme was cloned from the Escherichia coli genome by PCR based on the published partial sequence of the gene. The enzyme was overexpressed and purified to near homogeneity in two chromatographic steps and was analyzed kinetically in the forward and reverse directions. The Km...
保存先:
| 出版年: | Appl Environ Microbiol |
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| 主要な著者: | , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
American Society for Microbiology (ASM)
1997
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168564/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9212416/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.7.2695-2701.1997 |
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