Molecular epidemiological survey of rotaviruses in sewage by reverse transcriptase seminested PCR and restriction fragment length polymorphism assay.

Rotavirus double-stranded RNA was detected directly in sewage treatment plant samples over a 1-year period by reverse transcription followed by PCR amplification of the VP7 gene and Southern blot hybridization. The presence of naturally occurring rotaviruses was demonstrated in 42% of raw sewage sam...

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Bibliographic Details
Main Authors: Dubois, E, Le Guyader, F, Haugarreau, L, Kopecka, H, Cormier, M, Pommepuy, M
Format: Artigo
Language:Inglês
Published: 1997
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC168473/
https://ncbi.nlm.nih.gov/pubmed/9143113
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