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Molecular epidemiological survey of rotaviruses in sewage by reverse transcriptase seminested PCR and restriction fragment length polymorphism assay.

Rotavirus double-stranded RNA was detected directly in sewage treatment plant samples over a 1-year period by reverse transcription followed by PCR amplification of the VP7 gene and Southern blot hybridization. The presence of naturally occurring rotaviruses was demonstrated in 42% of raw sewage sam...

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Udgivet i:Appl Environ Microbiol
Principais autores: Dubois, E, Le Guyader, F, Haugarreau, L, Kopecka, H, Cormier, M, Pommepuy, M
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168473/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9143113/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.5.1794-1800.1997
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