Molecular cloning, expression, and characterization of a functional single-chain Fv antibody to the mycotoxin zearalenone.
The heavy-chain and kappa light-chain variable region genes of an antizearalenone hybridoma cell line (2G3-6E3-2E2) were isolated by PCR and joined by a DNA linker encoding peptide (Gly4Ser)3 as a single-chain Fv (scFv) DNA fragment. The scFv DNA fragment was cloned into a phagemid (pCANTAB5E) and e...
Guardat en:
| Publicat a: | Appl Environ Microbiol |
|---|---|
| Autors principals: | , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1997
|
| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168318/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8979354/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.1.263-269.1997 |
| Etiquetes: |
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
|