Molecular cloning, expression, and characterization of a functional single-chain Fv antibody to the mycotoxin zearalenone.

The heavy-chain and kappa light-chain variable region genes of an antizearalenone hybridoma cell line (2G3-6E3-2E2) were isolated by PCR and joined by a DNA linker encoding peptide (Gly4Ser)3 as a single-chain Fv (scFv) DNA fragment. The scFv DNA fragment was cloned into a phagemid (pCANTAB5E) and e...

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Hauptverfasser: Yuan, Q, Clarke, J R, Zhou, H R, Linz, J E, Pestka, J J, Hart, L P
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1997
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC168318/
https://ncbi.nlm.nih.gov/pubmed/8979354
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