Molecular cloning, expression, and characterization of a functional single-chain Fv antibody to the mycotoxin zearalenone.

The heavy-chain and kappa light-chain variable region genes of an antizearalenone hybridoma cell line (2G3-6E3-2E2) were isolated by PCR and joined by a DNA linker encoding peptide (Gly4Ser)3 as a single-chain Fv (scFv) DNA fragment. The scFv DNA fragment was cloned into a phagemid (pCANTAB5E) and e...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Yuan, Q, Clarke, J R, Zhou, H R, Linz, J E, Pestka, J J, Hart, L P
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168318/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8979354/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.1.263-269.1997
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