A PCR-based assay for the detection of Escherichia coli Shiga-like toxin genes in ground beef.
A detection system based on the PCR has been developed for Escherichia coli strains which harbor the Shiga-like toxin genes. This quantitative detection system involves the 5'-->3' nuclease activity of Thermus aquaticus DNA polymerase, which cleaves an internal oligonucleotide probe that has been la...
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| Publicado no: | Appl Environ Microbiol |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1996
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167901/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8919796/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.62.4.1347-1353.1996 |
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