A PCR-based assay for the detection of Escherichia coli Shiga-like toxin genes in ground beef.

A detection system based on the PCR has been developed for Escherichia coli strains which harbor the Shiga-like toxin genes. This quantitative detection system involves the 5'-->3' nuclease activity of Thermus aquaticus DNA polymerase, which cleaves an internal oligonucleotide probe that has been la...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Principais autores:	Witham, P K, Yamashiro, C T, Livak, K J, Batt, C A
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 1996
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167901/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8919796/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.62.4.1347-1353.1996
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