Cloning and characterization of the genes encoding the malolactic enzyme and the malate permease of Leuconostoc oenos.
Using degenerated primers from conserved regions of the protein sequences of malic enzymes, we amplified a 324-bp DNA fragment by PCR from Leuconostoc oenos and used this fragment as a probe for screening a Leuconostoc oenos genomic bank. Of the 2,990 clones in the genomic bank examined, 7 with over...
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| 發表在: | Appl Environ Microbiol |
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| Principais autores: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Microbiology (ASM)
1996
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167893/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8919788/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.62.4.1274-1282.1996 |
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