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Heteromeric MAPPIT: a novel strategy to study modification-dependent protein–protein interactions in mammalian cells

We recently reported a two-hybrid trap for detecting protein–protein interactions in intact mammalian cells (MAPPIT). The bait protein was fused to a STAT recruitment-deficient, homodimeric cytokine receptor and the prey protein to functional STAT recruitment sites. In such a configuration, STAT-dep...

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Vydáno v:Nucleic Acids Res
Hlavní autoři: Lemmens, Irma, Eyckerman, Sven, Zabeau, Lennart, Catteeuw, Dominiek, Vertenten, Els, Verschueren, Kristin, Huylebroeck, Danny, Vandekerckhove, Joël, Tavernier, Jan
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2003
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167658/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12853652/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng075
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