Heteromeric MAPPIT: a novel strategy to study modification-dependent protein–protein interactions in mammalian cells

We recently reported a two-hybrid trap for detecting protein–protein interactions in intact mammalian cells (MAPPIT). The bait protein was fused to a STAT recruitment-deficient, homodimeric cytokine receptor and the prey protein to functional STAT recruitment sites. In such a configuration, STAT-dep...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Lemmens, Irma, Eyckerman, Sven, Zabeau, Lennart, Catteeuw, Dominiek, Vertenten, Els, Verschueren, Kristin, Huylebroeck, Danny, Vandekerckhove, Joël, Tavernier, Jan
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2003
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167658/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12853652/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng075
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