Heteromeric MAPPIT: a novel strategy to study modification-dependent protein–protein interactions in mammalian cells

We recently reported a two-hybrid trap for detecting protein–protein interactions in intact mammalian cells (MAPPIT). The bait protein was fused to a STAT recruitment-deficient, homodimeric cytokine receptor and the prey protein to functional STAT recruitment sites. In such a configuration, STAT-dep...

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Detalles Bibliográficos
Main Authors: Lemmens, Irma, Eyckerman, Sven, Zabeau, Lennart, Catteeuw, Dominiek, Vertenten, Els, Verschueren, Kristin, Huylebroeck, Danny, Vandekerckhove, Joël, Tavernier, Jan
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2003
Assuntos:
NAR Methods Online
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC167658/
https://ncbi.nlm.nih.gov/pubmed/12853652
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