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Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water.

A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially...

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Detalles Bibliográficos
Main Authors: Arias, C R, Garay, E, Aznar, R
Formato: Artigo
Idioma:Inglês
Publicado: 1995
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC167627/
https://ncbi.nlm.nih.gov/pubmed/7574657
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