Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water.
A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially...
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| Udgivet i: | Appl Environ Microbiol |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1995
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167627/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7574657/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.9.3476-3478.1995 |
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