Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water.

A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Arias, C R, Garay, E, Aznar, R
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1995
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167627/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7574657/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.9.3476-3478.1995
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