Purification and characterization of the Bacillus subtilis levanase produced in Escherichia coli.
The enzyme levanase encoded by the sacC gene from Bacillus subtilis was overexpressed in Escherichia coli with the strong, inducible tac promoter. The enzyme was purified from crude E. coli cell lysates by salting out with ammonium sulfate and chromatography on DEAE-Sepharose CL-6B, S-Sepharose, and...
Gardado en:
| Publicado en: | Appl Environ Microbiol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1995
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167457/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7646030/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.5.1953-1958.1995 |
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