Purification and characterization of the Bacillus subtilis levanase produced in Escherichia coli.

The enzyme levanase encoded by the sacC gene from Bacillus subtilis was overexpressed in Escherichia coli with the strong, inducible tac promoter. The enzyme was purified from crude E. coli cell lysates by salting out with ammonium sulfate and chromatography on DEAE-Sepharose CL-6B, S-Sepharose, and...

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Vydáno v:	Appl Environ Microbiol
Hlavní autoři:	Wanker, E, Huber, A, Schwab, H
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1995
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167457/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7646030/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.5.1953-1958.1995
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