Purification and characterization of the Bacillus subtilis levanase produced in Escherichia coli.
The enzyme levanase encoded by the sacC gene from Bacillus subtilis was overexpressed in Escherichia coli with the strong, inducible tac promoter. The enzyme was purified from crude E. coli cell lysates by salting out with ammonium sulfate and chromatography on DEAE-Sepharose CL-6B, S-Sepharose, and...
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| Publicado no: | Appl Environ Microbiol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1995
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167457/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7646030/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.5.1953-1958.1995 |
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