Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers.
A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeri...
Gardado en:
| Publicado en: | Appl Environ Microbiol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1995
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167343/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7574620/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.2.817-819.1995 |
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