High accuracy genotyping directly from genomic DNA using a rolling circle amplification based assay

BACKGROUND: Rolling circle amplification of ligated probes is a simple and sensitive means for genotyping directly from genomic DNA. SNPs and mutations are interrogated with open circle probes (OCP) that can be circularized by DNA ligase when the probe matches the genotype. An amplified detection si...

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Pubblicato in:	BMC Genomics
Autori principali:	Alsmadi, Osama A, Bornarth, Carole J, Song, Wanmin, Wisniewski, Michele, Du, Jing, Brockman, Joel P, Faruqi, A Fawad, Hosono, Seiyu, Sun, Zhenyu, Du, Yuefen, Wu, Xiaohong, Egholm, Michael, Abarzúa, Patricio, Lasken, Roger S, Driscoll, Mark D
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	BMC 2003
Soggetti:	Methodology Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC165428/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12777185/ https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1471-2164-4-21
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