Development of a Duplex PCR Assay for Detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in Pig Feces

A duplex PCR (D-PCR) amplifying portions of the Brachyspira hyodysenteriae NADH oxidase gene and the B. pilosicoli 16S rRNA gene was developed and then tested on DNA extracted from 178 porcine fecal samples. The feces also underwent anaerobic culture and species-specific PCRs. Fecal extraction-D-PCR...

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Pubblicato in:	J Clin Microbiol
Autori principali:	La, Tom, Phillips, Nyree D., Hampson, David J.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2003
Soggetti:	Clinical Veterinary Microbiology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC165297/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12843096/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.7.3372-3375.2003
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