Development of a Duplex PCR Assay for Detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in Pig Feces

A duplex PCR (D-PCR) amplifying portions of the Brachyspira hyodysenteriae NADH oxidase gene and the B. pilosicoli 16S rRNA gene was developed and then tested on DNA extracted from 178 porcine fecal samples. The feces also underwent anaerobic culture and species-specific PCRs. Fecal extraction-D-PCR...

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Dades bibliogràfiques

Publicat a:	J Clin Microbiol
Autors principals:	La, Tom, Phillips, Nyree D., Hampson, David J.
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 2003
Matèries:	Clinical Veterinary Microbiology
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC165297/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12843096/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.7.3372-3375.2003
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