Development of a Duplex PCR Assay for Detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in Pig Feces

A duplex PCR (D-PCR) amplifying portions of the Brachyspira hyodysenteriae NADH oxidase gene and the B. pilosicoli 16S rRNA gene was developed and then tested on DNA extracted from 178 porcine fecal samples. The feces also underwent anaerobic culture and species-specific PCRs. Fecal extraction-D-PCR...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	La, Tom, Phillips, Nyree D., Hampson, David J.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2003
Assuntos:	Clinical Veterinary Microbiology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC165297/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12843096/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.7.3372-3375.2003
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