Recombinant Single-Chain Variable Fragment Antibodies Directed against Clostridium difficile Toxin B Produced by Use of an Optimized Phage Display System

Recombinant antibody cloning and phage display technologies were used to produce single-chain antibodies (scFv) against Clostridium difficile toxin B. The starting material was the mouse B cell hybridoma line 5A8, which generates a monoclonal antibody against the toxin. The integrated cloning, scree...

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Autori principali: Deng, Xiao K., Nesbit, Lance A., Morrow, Jr., K. John
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2003
Soggetti:
Microbial Immunology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC164272/
https://ncbi.nlm.nih.gov/pubmed/12853390
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CDLI.10.4.587-595.2003
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