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Characterization of Saccharomyces cerevisiae CYP51 and a CYP51 fusion protein with NADPH cytochrome P-450 oxidoreductase expressed in Escherichia coli.

Saccharomyces cerevisiae CYP51, target of azole antifungal agents, and CYP51 fused with S. cerevisiae cytochrome P-450 oxidoreductase (FUS protein) were expressed in active forms in Escherichia coli by cloning into pET15b. The expression was monitored immunologically, catalytically, and by using red...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
הוצא לאור ב:Antimicrob Agents Chemother
Principais autores: Venkateswarlu, K, Kelly, D E, Kelly, S L
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology (ASM) 1997
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC163793/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9087488/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.41.4.776
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