Systematic characterization of 2′-deoxynucleoside- 5′-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA

We synthesized C5-modified analogs of 2′-deoxyuridine triphosphate and 2′-deoxycytidine triphosphate and investigated them as substrates for PCRs using Taq, Tth, Vent(exo-), KOD Dash and KOD(exo-) polymerases and pUC 18 plasmid DNA as a template. These assays were performed on two different amplifyi...

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Hlavní autoři: Kuwahara, Masayasu, Nagashima, Jun-ichi, Hasegawa, Masatoshi, Tamura, Takehiro, Kitagata, Rina, Hanawa, Kazuo, Hososhima, Shin-ichi, Kasamatsu, Toshiyuki, Ozaki, Hiroaki, Sawai, Hiroaki
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2006
Témata:
Molecular Biology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1636466/
https://ncbi.nlm.nih.gov/pubmed/17012278
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl637
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