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Purification of a 54-kilodalton protein (OprJ) produced in NfxB mutants of Pseudomonas aeruginosa and production of a monoclonal antibody specific to OprJ.

The 54-kDa outer membrane protein (designated OprJ) of a norfloxacin-resistant nfxB mutant of Pseudomonas aeruginosa PAO1 was purified by ion-exchange high-performance liquid chromatography. Mobility of OprJ in sodium dodecyl sulfate-polyacrylamide gel electrophoresis was not affected by reduction a...

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Detalhes bibliográficos
Publicado no:Antimicrob Agents Chemother
Principais autores: Hosaka, M, Gotoh, N, Nishino, T
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC162817/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7486910/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.39.8.1731
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