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Shotgun sequencing of the human transcriptome with ORF expressed sequence tags
Theoretical considerations predict that amplification of expressed gene transcripts by reverse transcription–PCR using arbitrarily chosen primers will result in the preferential amplification of the central portion of the transcript. Systematic, high-throughput sequencing of such products would resu...
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Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
The National Academy of Sciences
2000
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC16267/ https://ncbi.nlm.nih.gov/pubmed/10737800 |
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