Shotgun sequencing of the human transcriptome with ORF expressed sequence tags

Theoretical considerations predict that amplification of expressed gene transcripts by reverse transcription–PCR using arbitrarily chosen primers will result in the preferential amplification of the central portion of the transcript. Systematic, high-throughput sequencing of such products would resu...

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Publicado en:	Proc Natl Acad Sci U S A
Autores principales:	Dias Neto, Emmanuel, Garcia Correa, Ricardo, Verjovski-Almeida, Sergio, Briones, Marcelo R. S., Nagai, Maria Aparecida, da Silva, Wilson, Zago, Marco Antonio, Bordin, Silvana, Costa, Fernando Ferreira, Goldman, Gustavo Henrique, Carvalho, Alex F., Matsukuma, Adriana, Baia, Gilson S., Simpson, David H., Brunstein, Adriana, de Oliveira, Paulo S. L., Bucher, Philipp, Jongeneel, C. Victor, O'Hare, Michael J., Soares, Fernando, Brentani, Ricardo R., Reis, Luis F. L., de Souza, Sandro J., Simpson, Andrew J. G.
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	National Academy of Sciences 2000
Materias:	Biological Sciences
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC16267/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10737800/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.7.3491
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