Shotgun sequencing of the human transcriptome with ORF expressed sequence tags

Theoretical considerations predict that amplification of expressed gene transcripts by reverse transcription–PCR using arbitrarily chosen primers will result in the preferential amplification of the central portion of the transcript. Systematic, high-throughput sequencing of such products would resu...

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Εκδόθηκε σε:	Proc Natl Acad Sci U S A
Κύριοι συγγραφείς:	Dias Neto, Emmanuel, Garcia Correa, Ricardo, Verjovski-Almeida, Sergio, Briones, Marcelo R. S., Nagai, Maria Aparecida, da Silva, Wilson, Zago, Marco Antonio, Bordin, Silvana, Costa, Fernando Ferreira, Goldman, Gustavo Henrique, Carvalho, Alex F., Matsukuma, Adriana, Baia, Gilson S., Simpson, David H., Brunstein, Adriana, de Oliveira, Paulo S. L., Bucher, Philipp, Jongeneel, C. Victor, O'Hare, Michael J., Soares, Fernando, Brentani, Ricardo R., Reis, Luis F. L., de Souza, Sandro J., Simpson, Andrew J. G.
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	National Academy of Sciences 2000
Θέματα:	Biological Sciences
Διαθέσιμο Online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC16267/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10737800/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.7.3491
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