Shotgun sequencing of the human transcriptome with ORF expressed sequence tags

Theoretical considerations predict that amplification of expressed gene transcripts by reverse transcription–PCR using arbitrarily chosen primers will result in the preferential amplification of the central portion of the transcript. Systematic, high-throughput sequencing of such products would resu...

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書誌詳細

出版年:	Proc Natl Acad Sci U S A
主要な著者:	Dias Neto, Emmanuel, Garcia Correa, Ricardo, Verjovski-Almeida, Sergio, Briones, Marcelo R. S., Nagai, Maria Aparecida, da Silva, Wilson, Zago, Marco Antonio, Bordin, Silvana, Costa, Fernando Ferreira, Goldman, Gustavo Henrique, Carvalho, Alex F., Matsukuma, Adriana, Baia, Gilson S., Simpson, David H., Brunstein, Adriana, de Oliveira, Paulo S. L., Bucher, Philipp, Jongeneel, C. Victor, O'Hare, Michael J., Soares, Fernando, Brentani, Ricardo R., Reis, Luis F. L., de Souza, Sandro J., Simpson, Andrew J. G.
フォーマット:	Artigo
言語:	Inglês
出版事項:	National Academy of Sciences 2000
主題:	Biological Sciences
オンライン･アクセス:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC16267/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10737800/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.7.3491
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