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Glutamine Synthetase GlnA1 Is Essential for Growth of Mycobacterium tuberculosis in Human THP-1 Macrophages and Guinea Pigs

To assess the role of glutamine synthetase (GS), an enzyme of central importance in nitrogen metabolism, in the pathogenicity of Mycobacterium tuberculosis, we constructed a glnA1 mutant via allelic exchange. The mutant had no detectable GS protein or GS activity and was auxotrophic for l-glutamine....

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Detalles Bibliográficos
Main Authors: Tullius, Michael V., Harth, Günter, Horwitz, Marcus A.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2003
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC162033/
https://ncbi.nlm.nih.gov/pubmed/12819079
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.71.7.3927-3936.2003
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