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Rhodopsin kinase: Expression in mammalian cells and a two-step purification

A suitable system for expression of the rhodopsin kinase (RK) gene and its mutants is needed for structure–function studies of RK. Previously, investigation of the baculovirus system showed satisfactory production of RK, but posttranslational isoprenylation was deficient. We now report on a comparat...

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Autors principals: Bruel, Christophe, Cha, Kiweon, Reeves, Philip J., Getmanova, Elena, Khorana, H. Gobind
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 2000
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC16182/
https://ncbi.nlm.nih.gov/pubmed/10737781
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