NMR spectroscopy in studies of light-induced structural changes in mammalian rhodopsin: Applicability of solution (19)F NMR

We report high resolution solution (19)F NMR spectra of fluorine-labeled rhodopsin mutants in detergent micelles. Single cysteine substitution mutants in the cytoplasmic face of rhodopsin were labeled by attachment of the trifluoroethylthio (TET), CF(3)-CH(2)-S, group through a disulfide linkage. TE...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Klein-Seetharaman, Judith, Getmanova, Elena V., Loewen, Michèle C., Reeves, Philip J., Khorana, H. Gobind
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1999
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC24135/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10570143/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.24.13744
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