NMR spectroscopy in studies of light-induced structural changes in mammalian rhodopsin: Applicability of solution (19)F NMR

We report high resolution solution (19)F NMR spectra of fluorine-labeled rhodopsin mutants in detergent micelles. Single cysteine substitution mutants in the cytoplasmic face of rhodopsin were labeled by attachment of the trifluoroethylthio (TET), CF(3)-CH(2)-S, group through a disulfide linkage. TE...

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Main Authors: Klein-Seetharaman, Judith, Getmanova, Elena V., Loewen, Michèle C., Reeves, Philip J., Khorana, H. Gobind
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1999
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC24135/
https://ncbi.nlm.nih.gov/pubmed/10570143
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